Download ORF8 is one of the most variable among SARS-CoV-1. Remarkably, 29 nucleotide N-terminal sequence has gone under gradual deletion during SARS-CoV-1 spread in humans. Early tests showed a decrease in viral replication by up to 23-fold when this sequence was removed. SARS-CoV-2 ORF8 is 127 amino acids long and 45% identical to SARS-CoV-1 ORF8b. […]
Download Nucleocapsid (N) protein packages the viral RNA into a helical ribonucleocapsid and plays a key role in viral assembly. SARS-CoV-2 N protein is a 419 amino acid long protein and is 89% identical to SARS-CoV-1 nucleocapsid. The RNA binding domain of N is located at the N-terminal and all these residues are fully conserved […]
Download ORF9 is an accessory protein synthesized from an alternative reading frame in the N gene. At mitochondrial level, ORF9 promotes suppression of the activation of INF regulatory factors and NF-κB. ORF9 forms a symmetric dimer where the monomer interactions resemble a handshake. Dimer assembling creates a hydrophobic tunnel that can accomodate a long fatty […]
Download Within SARS-COV-2, ORF10 is thought to not have any functional protein purpose as it was not found within sub genomic mRNA sequencing. In another study, ORF10 might hijack components of the ubiquitination machinery and degradation. ORF10 of SARS-COV-2 is the last and the shortest predicted coding sequence upstream of the poly-A tail. ORF10 is […]
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